Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner.
Zhang P, Rausch C, Hastert FD, Boneva B, Filatova A, Patil SJ, Nuber UA, Gao Y, Zhao X, Cardoso MC
Nucleic Acids Res. 2017 Apr 25. doi: 10.1093/nar/gkx281.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167563 | pENeCFPCNAL2 pc922 | For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA). |
| 246848 | pCAG-RFP-mouse Tet1 (pc2329) | Expresses mRFP tagged mouse Tet1 in mammalian cells. N-terminal tagged to mCherry. |
| 246849 | pmMBD1.2G (pc2845) | Mammalian expression plasmid of mouse MBD1 N-terminus (aa396-636). C-terminal tagged to GFP. |
| 246850 | pmGMBD1.3(pc2899) | Mammalian expression plasmid of mouse MBD1 lacking the CXXC3 domain. N-terminal tagged to GFP. |
| 246851 | pGmMBD1.4 (pc3305) | Mammalian expression plasmid of mouse MBD1 lacking the MBD domain (aa 75–636). N-terminal tagged to GFP. |
| 246852 | pGmMBD1.5 (pc3306) | Mammalian expression plasmid of MBD domain and NLS of mouse MBD1. N-terminal tagged to GFP. |
| 246862 | pCAG-mch-Tet2CD (pc3338) | Expresses mCherry-tagged catalytic active domain of mouse Tet2 in mammalian cells. N-terminal tagged to mCherry. |
| 246869 | pCAG-mch-Tet3CD (pc3339) | Expresses mCherry-tagged catalytic active domain of mouse Tet3 in mammalian cells. N-terminal tagged to mCherry. |
| 246870 | pmMBD1.7G (pc3343) | Mammalian expression plasmid of full length MBD1 with pointmutations C289 and 292A. C-terminal tagged to GFP |