Conformational engineering of HIV-1 Env based on mutational tolerance in the CD4 and PG16 bound states.
Heredia JD, Park J, Choi H, Gill KS, Procko E
J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19. PubMed Article
Heredia JD, Park J, Choi H, Gill KS, Procko E
J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100918 | pCEP4-intron-BaLgp160 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate |
| 100919 | pCEP4-BaLgp160 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate |
| 100921 | pCEP4-BaLgp160-QES.01 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 100923 | pCEP4-BaLgp160-QES.02 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 100924 | pcDNA3-BaLgp140-8his | Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain |
| 100925 | pcDNA3-BaLgp120-8his | Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain |
| 100926 | pCEP4-DU422gp160 | Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate |
| 100927 | pCEP4-YU2gp160 | Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate |
| 100928 | pCEP4-Q769gp160 | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain |
| 100929 | pcDNA3-Q769gp120-8his | Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain |
| 100930 | pCEP4-Q769gp160-QES.03 | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16 |
| 100931 | pCEP4-Q842gp160 | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain |
| 100932 | pcDNA3-Q842gp120-8his | Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain |
| 100933 | pCEP4-Q842gp160-QES.04 | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16 |
| 111837 | pCEP4-Q842gp160-QES.i04.I181L | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16 |
| 111838 | pCEP4-Q769gp160-QES.i03.V255M | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16 |
| 111839 | pCEP4-BaLgp160-QES.i01.c01 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 111840 | pCEP4-DU422gp160-QES.c03 | Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 111841 | pCEP4-25711gp160 | Mammalian expression plasmid for Env from the 25711 HIV-1 strain |
| 111842 | pCEP4-25711gp160-QES.c02 | Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 111843 | pCEP4-BaLgp140-TM | Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether |
| 111844 | pCEP4-BG505.SOSIP-TM | Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether |
| 111845 | pCEP4-BG505.SOSIP-QES.i03.c01-TM | Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16 |
| 111846 | pCEP4-BG505.SOSIP-QES.i03.c01-8his | Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16 |
| 111847 | pCEP4-BG505.SOSIP-8his | Mammalian expression plasmid for soluble BG505 SOSIP.664 |
| 123212 | pCEP4-BaLgp160-754* | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation |
| 123213 | pCEP4-BaL-QES.i01.c08-754* | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123214 | pCEP4-BaL-QES.i01.c08 | Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123217 | pCEP4-25711gp160-754* | Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation |
| 123218 | pCEP4-25711-QES.c11-754* | Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123219 | pCEP4-25711-QES.c11 | Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123220 | pCEP4-ZM197M-gp160-754* | Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation |
| 123222 | pCEP4-ZM197M-QES.c10-754* | Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123224 | pCEP4-ZM197M-QES.c10 | Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123226 | pCEP4-ZM197M-gp160 | Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate |
| 123227 | pCEP4-Q769gp160-754* | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; C-terminal truncation |
| 123228 | pCEP4-Q769-QES.i03.c04-754* | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123229 | pCEP4-Q769-QES.i03.c04 | Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123231 | pCEP4-Q842gp160-754* | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation |
| 123232 | pCEP4-Q842-QES.i04.c05-754* | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123233 | pCEP4-Q842-QES.i04.c05 | Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123234 | pCEP4-191084-gp160 | Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate |
| 123236 | pCEP4-191084-gp160-754* | Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation |
| 123238 | pCEP4-191084-QES.i06.c07-754* | Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123239 | pCEP4-191084-QES.i06.c07 | Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123240 | pCEP4-B41gp160-754* | Mammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation |
| 123244 | pCEP4-B41gp160-V200E-754* | Mammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123246 | pCEP4-B41gp160-V200E | Mammalian expression plasmid for Env from the B41 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123247 | pCEP4-B41gp160 | Mammalian expression plasmid for Env from the B41 HIV-1 isolate |
| 123248 | pCEP4-DU422gp160-754* | Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncation |
| 123249 | pCEP4-DU422-QES.c12-754* | Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123250 | pCEP4-DU422-QES.c12 | Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123251 | pCEP4-DU422gp140-TM | Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether |
| 123252 | pCEP4-001428gp160-754* | Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation |
| 123253 | pCEP4-001428-QES.c10.L544Y-754* | Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16 |
| 123254 | pCEP4-001428-QES.c10.L544Y | Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; QES mutant with enhanced binding to PG16 |
| 123255 | pCEP4-001428gp160 | Mammalian expression plasmid for Env from the 001428 HIV-1 isolate |
| 123256 | pCEP4-001428(753)-VC | Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC) |
| 123257 | pCEP4-AD8gp160-754* | Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation |
| 123258 | pCEP4-AD8-QES.i08.c04-754* | Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes |
| 123259 | pCEP4-AD8-QES.i08.c04 | Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes |
| 123260 | pCEP4-AD8gp160 | Mammalian expression plasmid for Env from the AD8 HIV-1 isolate |
| 123263 | pCEP4-CH111gp160-754* | Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation |
| 123264 | pCEP4-CH111-QES.c13.L544Y-754* | Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes |
| 123267 | pCEP4-CH111-QES.c13.L544Y | Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes |
| 123268 | pCEP4-CH111gp160 | Mammalian expression plasmid for Env from the CH111 HIV-1 isolate |
| 123269 | pCEP4-SHIV327C-HuA10-QES.i09.c07 | Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes |
| 123270 | pCEP4-SHIV327C-HuA10-gp160 | Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain |
| 123271 | pCEP4-H031-QES.i07.c09 | Mammalian expression plasmid for Env from the H031 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes |
| 123272 | pCEP4-H031gp160 | Mammalian expression plasmid for Env from the H031 HIV-1 isolate |
| 123273 | pCEP4-BG505.SOSIP-gp160 | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations) |
| 123274 | pCEP4-BG505.SOSIP-gp160-754* | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation |
| 123275 | pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754* | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes |
| 123276 | pCEP4-BG505.SOSIP-gp160-QES.i05.c06 | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes |
| 123277 | pCEP4-BG505.DS-SOSIP.4mut-gp160 | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variant |
| 123278 | pCEP4-BG505.DS-SOSIP.6mut-gp160 | Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant |
| 123279 | pCEP4-CH115gp160-QES.c14 | Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes |
| 123280 | pCEP4-CH115gp160 | Mammalian expression plasmid for Env from the CH115 HIV-1 isolate |
| 123281 | pCEP4-CH115gp160-754* | Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation |
| 123282 | pCEP4-CH115gp160-QES.c14-754* | Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes |
| 123283 | pCMV-MA-VN | Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN) |