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Dynamics and consequences of spliceosome E complex formation.
Larson JD, Hoskins AA
Elife. 2017 Aug 22;6. pii: 27592. doi: 10.7554/eLife.27592. PubMed Article

Plasmids from Article

ID Plasmid Purpose
100986RP51A-NoBS-NoPseudoBSRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS
100987RP51A-No5'ss-NoBS-NoPseudoBSRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS
100988Rp51a Hyperstabilized 5'SSpUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT
100989Rp51a_10bp 5'SS_No BP or pBP_pUC18Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
100990Rp51a_SUS1 5'ss_No_BP_pBP-pUC18Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
100991Rp51a_SUS1 5'SSContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).
100992Rp51a_No_BP-pUC18Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

Antibodies from Article