Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control.
Baumschlager A, Aoki SK, Khammash M
ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 10.1021/acssynbio.7b00169. Epub 2017 Oct 18. PubMed Article
Baumschlager A, Aoki SK, Khammash M
ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 10.1021/acssynbio.7b00169. Epub 2017 Oct 18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101661 | pAB150 | Opto-T7RNAP*(563) |
| 101662 | pAB147 | Opto-T7RNAP*(302) |
| 101663 | pAB152 | Opto-T7RNAP*(563-F2) |
| 101664 | pAB153 | Opto-T7RNAP*(600) |
| 101665 | pAB144 | Opto-T7RNAP*(69) |
| 101666 | pAB151 | Opto-T7RNAP*(563-F1) |
| 101667 | pAB164 | Opto-T7RNAP*(563), half expr. |
| 101668 | pAB166 | Opto-T7RNAP*(563-F2), half expr. |
| 101669 | pAB170 | Opto-T7RNAP*(69), half expr. |
| 101670 | pAB171 | Opto-T7RNAP*(69), equal expr. |
| 101671 | pAB168 | Opto-T7RNAP*(563-F1), equal expr. |
| 101672 | pAB167 | Opto-T7RNAP*(563), equal expr. |
| 101673 | pAB174 | Opto-T7RNAP*(179) |
| 101674 | pAB202 | Opto-T7RNAP(563) |
| 101675 | pAB203 | Opto-T7RNAP(563-F1) |
| 101676 | pSKA322 | empty backbone with chloramphenicol resistance |
| 101677 | pAB213 | mCherry under T7 promoter control |
| 101678 | pAB50 | mCherry under T7 promoter control |
| 101679 | pAB143 | LAA-tagged mCherry under T7 promoter control |