Histidine catabolism is a major determinant of methotrexate sensitivity.
Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 10.1038/s41586-018-0316-7. PubMed Article
Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 10.1038/s41586-018-0316-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102312 | pLentiCRISPR sgFTCD_1 | genetic depletion of FTCD |
| 102313 | pLentiCRISPR sgFTCD_2 | genetic depletion of FTCD |
| 102314 | pLentiCRISPR sgSLC19A1 | genetic depletion of SLC19A1 |
| 102315 | pLentiCRISPR sgHAL | genetic depletion of HAL |
| 102316 | pLentiCRISPR sgAMDHD1 | genetic depletion of AMDHD1 |
| 102317 | pWP1_mFTCD | expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2 |
| 102318 | pWP1_mHAL | expression of mouse cDNA of HAL refractory to the guide in plasmid 4 |
| 102320 | FUW-RLuc-T2A-PuroR | expression of r-luciferase |