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Histidine catabolism is a major determinant of methotrexate sensitivity.
Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 10.1038/s41586-018-0316-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102312pLentiCRISPR sgFTCD_1genetic depletion of FTCD
102313pLentiCRISPR sgFTCD_2genetic depletion of FTCD
102314pLentiCRISPR sgSLC19A1genetic depletion of SLC19A1
102315pLentiCRISPR sgHALgenetic depletion of HAL
102316pLentiCRISPR sgAMDHD1genetic depletion of AMDHD1
102317pWP1_mFTCDexpression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2
102318pWP1_mHALexpression of mouse cDNA of HAL refractory to the guide in plasmid 4
102320FUW-RLuc-T2A-PuroRexpression of r-luciferase