Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish.
Chong-Morrison V, Mayes S, Simoes FC, Senanayake U, Carroll DS, Riley PR, Wilson SW, Sauka-Spengler T
Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. Epub 2023 Jun 27. PubMed Article
Chong-Morrison V, Mayes S, Simoes FC, Senanayake U, Carroll DS, Riley PR, Wilson SW, Sauka-Spengler T
Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. Epub 2023 Jun 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102416 | pVC-Ds-MCS-Ds | Empty vector with a multiple cloning site flanked by Ds integration elements |
| 102417 | pVC-Ds-E1b:eGFP-Ds | Fluorescent vector for screening enhancer elements in zebrafish |
| 102418 | pAC-SP6 | Ac transposase with SP6 promoter for in vitro transcription |
| 119059 | pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds | Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity) |
| 119061 | pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2 | Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version |
| 119065 | pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT | Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination |
| 119067 | pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT | Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination |
| 119069 | pVC-Ds-DrU6a:sgRNA-Ds | To clone sgRNA spacer sequence for microinjections in zebrafish |
| 119070 | pVC-Ds-DrU6a:scrambled1-sgRNA-Ds | Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish |
| 119071 | pVC-Ds-DrU6a:scrambled2-sgRNA-Ds | Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish |
| 119072 | pVC-Ds-DrU6a:scrambled3-sgRNA-Ds | Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish |
| 119073 | pVC-Ds-DrU6a:scrambled4-sgRNA-Ds | Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish |
| 119074 | pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds | Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter |
| 119075 | pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds | Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter |
| 119076 | pVC-Ds-DrU6a:sgRNA-MS2-Ds | To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops |
| 119077 | pVC-Ds-DrU6a:sgRNA-com-Ds | To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops |