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Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells.
Guo S, Murray RM
ACS Synth Biol. 2019 Mar 15;8(3):606-610. doi: 10.1021/acssynbio.8b00493. Epub 2019 Mar 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102449pSG13-pBAD-TetRssrAExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.
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102450pSG15-pBAD-tetO-deGFPExpresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.
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102451pSG22-pBAD-TetRExpresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.
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102452pSG25-pBAD-tetO-deGFPssrAExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.
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102453pSG28-J23151-AraCExpresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.
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102454pSG81-pLas-tetO-deGFPssrAExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.
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102455pSG84-pLas-TetRssrAExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.
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102456pSG86-J23151-LasRExpresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.
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Antibodies from Article