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Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A
Nature. 2017 Sep 7;549(7670):111-115. doi: 10.1038/nature23875. Epub 2017 Aug 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
91835pGL4.23-IL2RA CaRE3 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0612)
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91836pGL4.23-IL2RA CaRE5 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0613)
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91837pGL4.23-IL2RA CaRE1 (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0614)
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91838pGL4.23-IL2RA CaRE1 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0615)
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91839pGL4.23-IL2RA CaRE6 (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0616)
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91840pGL4.23-IL2RA CaRE6 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0617)
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91841pGL4.23-IL2RA CaRE5 (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0618)
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91842pGL4.23-CD69 CaRE1 (minus strand)Luciferase reporter for CD69 enhancer (IGI-P0619)
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91843pGL4.23-CD69 CaRE2 (minus strand)Luciferase reporter for CD69 enhancer (IGI-P0620)
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91844pGL4.23-CD69 CaRE3 (plus strand)Luciferase reporter for CD69 enhancer (IGI-P0621)
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91845pGL4.23-CD69 CaRE3 (minus strand)Luciferase reporter for CD69 enhancer (IGI-P0622)
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91846pGL4.23-IL2RA CaRE2 (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0623)
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91847pGL4.23-IL2RA CaRE2 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0624)
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91848pGL4.23-CD69 CaRE2 (plus strand)Luciferase reporter for CD69 enhancer (IGI-P0625)
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91849pGL4.23-IL2RA CaRE4 (minus strand)Luciferase reporter for IL2RA enhancer (IGI-P0626)
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91850pGL4.23-IL2RA CaRE4 (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0345)
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91851pGL4.23-IL2RA CaRE4 SNP (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0346)
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91852pGL4.23-IL2RA CaRE4 scramble (plus strand)Luciferase reporter for IL2RA enhancer (IGI-P0347)
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Antibodies from Article