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A modular toolbox for Golden-Gate-based plasmid assembly streamlines generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.
Wu D, Schandry N, Lahaye T
Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632.
PubMed Article

Plasmids from Article

ID Plasmid  
104514LI A-B phrpB
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104515LI A-B pEPS
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104516LI A-B pGala7
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104517LI C-D GUS
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104518LI C-D Dest
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104519LI D-E V5
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104520LI E-F Tfd
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104521LI F-G Spec
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104522LI F-G Gent
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104523LI F-G Kan
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104524LI F-G Tet
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104525LI F-G Cml
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104526LI Rsc202375 Upstream
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104527LI Rsc203337 Downstream
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104528LI LacZ
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104529LII destination
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104530LIII 1-6 Amp RC
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