Addgene: Multiplex recording of cellular events over time on CRISPR biological tape.

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Browse
Harris Wang
Sheth et al

Multiplex recording of cellular events over time on CRISPR biological tape.
Sheth RU, Yim SS, Wu FL, Wang HH
Science. 2017 Dec 15;358(6369):1457-1461. doi: 10.1126/science.aao0958. Epub 2017 Nov 23. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
104572	pRec	Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.
104573	pRec-GalS	Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively.
104574	pRec-TreR	Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.
104575	pRec ΔLacI	Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.
104576	pTrig	Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.
104578	pTrig-CopA	Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.

Antibodies from Article