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Membrane constriction and thinning by sequential ESCRT-III polymerization.
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol. 2020 Apr;27(4):392-399. doi: 10.1038/s41594-020-0404-x. Epub 2020 Apr 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
108284pGEX2T-TEV CHMP1B 4-178Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279
108285pGEX2T-TEV CHMP1B 186-199Expresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283
108286pGEX2T-TEV CHMP1B 1-143Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278
108287pGEX2T-TEV CHMP1B 144-178Expresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281
108288pGEX2T-TEV CHMP1B 186-199 L188A/L192AExpresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
108289pGEX2T-TEV CHMP1B 4-199 L188A/L192AExpresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
108290pGEX2T-TEV CHMP1B 186-199 L192A/L195AExpresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
108291pGEX2T-TEV CHMP1B 4-199 L192A/L195AExpresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
115325pCA528 CHMP1B 4-199Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23
115326pCA528 CHMP1B 1-199Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

Antibodies from Article