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Brigitte Gasser Lab: CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer,R.
(2018) In Press

Plasmids from Article

ID Plasmid Purpose  
104906BB3cN_pGAP_23*_pLAT1_Cas9hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
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104907BB3cH_pGAP_23*_pLAT1_Cas9hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
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104908BB3cK_pGAP_23*_pLAT1_Cas9hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
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104909BB3cK_pGAP_23*_pTEF_Cas9hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
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104910BB3cK_pGAP_23*_pPFK300_Cas9hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
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104911BB3cN_pGAP_23*_pPFK300_Cas9hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
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104912BB3cH_pGAP_23*_pPFK300_Cas9hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
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108686BB3nK_ext_ACPlasmid for assembly of donor DNA templates consisting of two expression units
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108687BB3nK_ext_ADPlasmid for assembly of donor DNA templates consisting of three expression units
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