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Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae.
Ferreira R, Skrekas C, Nielsen J, David F
ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
103139p413_Csy4NLSExpresses Csy4-NLS under TEF promoter
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103140dCas9v2TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site
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103141pCRA03Triple gRNA expression separated with 28nt Csy4 motif
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