Addgene: Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae.

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Jens Nielsen
Ferreira et al

Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae.
Ferreira R, Skrekas C, Nielsen J, David F
ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
103139	p413_Csy4NLS	Expresses Csy4-NLS under TEF promoter
103140	dCas9v2	TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site
103141	pCRA03	Triple gRNA expression separated with 28nt Csy4 motif

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