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Addgene
A simple and versatile system for the ATP-dependent assembly of chromatin.
Khuong MT, Fei J, Cruz-Becerra G, Kadonaga JT
J Biol Chem. 2017 Nov 24;292(47):19478-19490. doi: 10.1074/jbc.M117.815365. Epub 2017 Oct 5. PubMed Article

Plasmids from Article

ID Plasmid Purpose
105498pET21-His6dNLPExpression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein
105499pET24-ISWI-iCBDExpression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)
105500pET11a-dH2AExpression of Drosophila histone H2A
105501pET11a-dH2BExpression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level
105502pET11a-dH3-dH4Co-expression of Drosophila histones H3 and H4
105503pET-NDH6Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Antibodies from Article