Thanos Halazonetis Lab:
Expression plasmids for production of Rev7 and Champ1 proteins
Thanos Halazonetis
Unpublished
Thanos Halazonetis
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105630 | pT5T_Rev7_1-211_SA112_KVK_YAK | Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3 |
| 105634 | pT5T_Rev7_1-211_SA112_KVK | Expresses mouse monomeric Rev7 protein |
| 105635 | pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898 | Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid |
| 105637 | pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014 | Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid |
| 105638 | pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355 | Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid |
| 105639 | pIRESN2_GFP_YTA_Champ1_328-354 | Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP |
| 105672 | pCDZ2FE_Rev3_1873-2025 | Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein |
| 105673 | pCDZ2FE_Rev3_1873-2025_4PA | Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding |
| 105674 | pCDZ2FE_Pogz_1-1410 | Expresses Flag-tagged full-length human POGZ protein |
| 105675 | pCDZ2FE_Champ1_1-812 | Expresses Flag-tagged full-length human Champ1 protein |
| 105676 | pCDZ2FE_Champ1_1-812_2PA | Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding |