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FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling.
Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
85845pDONOR-SNCAe2-WTDonor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP
85847pDONOR-SNCAe3-WTDonor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato
85848pDONOR-SNCAe3-A53TDonor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP
86154pDONOR-PARK6e5-I368NDonor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato
100603pDONOR-tagBFP-PSM-EGFPDonor scaffold with EGFP positive selection module, and tagBFP negative selection module
100604pDONOR-tagBFP-PSM-dTOMATODonor scafold with dTOMATO positive selection module, and tagBFP negative selection module

Antibodies from Article