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Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells.
Serfling R, Lorenz C, Etzel M, Schicht G, Bottke T, Morl M, Coin I
Nucleic Acids Res. 2017 Nov 21. pii: 4641905. doi: 10.1093/nar/gkx1156.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105830pNEU-hMbPylRS-4xU6M15Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

Recombinant Antibodies from Article