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Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses.
Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert JS, Geeves M, Oertner TG, Torok K
Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105832pCMV iGluuFast indicator for extracellular glutamate imaging
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105833pCMV iGlufFast indicator for extracellular glutamate imaging
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106121pCI syn iGlufFast indicator for synaptic glutamate imaging (synapsin promoter)
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106122pCI syn iGluuFast indicator for synaptic glutamate imaging (synapsin promoter)
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106123pCI syn iGluSnFRIndicator for glutamate imaging (synapsin promoter)
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119828pET41a iGluSnFRProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli
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119829pET41a iGlumProduction of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli
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119830pET41a iGluhProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli
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119831pET41a iGluSnFR E25RProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli
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119832pET41a iGlulProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli
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119833pET41a iGlufProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli
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119834pET41a iGluuProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli
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119835pET30b GluBP600nProduction of recombinant GluBP600n high affinity protein in E. coli
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