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Interkinetic nuclear migration is centrosome independent and ensures apical cell division to maintain tissue integrity.
Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
Dev Cell. 2015 Jan 26;32(2):203-19. doi: 10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105940pCS2+ EB3-mKate2in vitro transcription
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105941pCS2+ centrin-mKate2in vitro transcription, zf centrin N-terminally tagged with mkate2
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105942HS-PCNA-GFPheat shock inducible transgenesis
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105943HS-DN-Cep152-mKate2heat shock inducible transgenesis
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105944HS-OS-Plk4-mKate2heat shock inducible transgenesis, non phoshorylable version of Plk4
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105945HS-mKate2heat shock inducible transgenesis, cytosolic mKate2
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105946HS-aPKC-CAAX-GFPheat shock inducible transgenesis
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105947HS-Par3-GFPheat shock inducible transgenesis
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105948HS-EB3-GFPheat shock inducible transgenesis
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105949HS-DN-Plk1heat shock inducible transgenesis
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105950HS-aPKC-CAAX-mKate2heat shock inducible transgenesis
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105951HS-Ras-mKate2heat shock inducible transgenesis
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105952HS-Utrophin-GFPheat shock inducible transgenesis
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105953HS-H2B-RFPheat shock inducible transgenesis
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105954HS-GFP-PACTheat shock inducible transgenesis, PACT is a centriol marker
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Antibodies from Article