Fluorescence-based quantitative synapse analysis for cell-type specific connectomics.
Kuljis DA, Park E, Telmer CA, Lee J, Ackerman DS, Bruchez MP, Barth AL
eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105981 | pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH | This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato. |
| 105982 | pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40 | This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato. |
| 105983 | pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40 | This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato. |