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pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40
(Plasmid #105982)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105982 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-FLEX
  • Backbone size w/o insert (bp) 4894
  • Total vector size (bp) 7240
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Plasmid is very unstable screen clones for recombination. Grow at 28oC if possible.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
  • Species
    H. sapiens (human), M. musculus (mouse), S. cerevisiae (budding yeast); Thosea asigna virus
  • Insert Size (bp)
    2346
  • Promoter human synapsin 1
  • Tags / Fusion Proteins
    • FAPdL5-POST (N terminal on insert)
    • T2A-dTomato (C terminal on insert)
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site KpnI partial (not destroyed)
  • 5′ sequencing primer hSynF
  • 3′ sequencing primer WPRErev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40 was a gift from Marcel Bruchez (Addgene plasmid # 105982 ; http://n2t.net/addgene:105982 ; RRID:Addgene_105982)
  • For your References section:

    Fluorescence-based quantitative synapse analysis for cell-type specific connectomics. Kuljis DA, Park E, Telmer CA, Lee J, Ackerman DS, Bruchez MP, Barth AL. eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019. 10.1523/ENEURO.0193-19.2019 PubMed 31548370