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pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH
(Plasmid #105981)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105981 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    PENN vector core
  • Backbone size w/o insert (bp) 4643
  • Total vector size (bp) 6984
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Highly susceptible to recombination, screen clones. Grow at 28oC if possible.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
  • Species
    H. sapiens (human), M. musculus (mouse), B. taurus (bovine), S. cerevisiae (budding yeast); Thosea asigna virus (T2A), Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)
  • Insert Size (bp)
    2341
  • Promoter human synapsin 1
  • Tags / Fusion Proteins
    • FAPdL5-POST (N terminal on insert)
    • T2A-dTomato (C terminal on insert)
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer hSynF
  • 3′ sequencing primer WPRErev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH was a gift from Marcel Bruchez (Addgene plasmid # 105981 ; http://n2t.net/addgene:105981 ; RRID:Addgene_105981)
  • For your References section:

    Fluorescence-based quantitative synapse analysis for cell-type specific connectomics. Kuljis DA, Park E, Telmer CA, Lee J, Ackerman DS, Bruchez MP, Barth AL. eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019. 10.1523/ENEURO.0193-19.2019 PubMed 31548370