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Time-gated detection of protein-protein interactions with transcriptional readout.

Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY
Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104839pAAV-UAS-CITRINESPARK reporter gene
104840pAAV-UAS-LuciferaseSPARK reporter gene
104841pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5b2AR-SPARK TF component
104842pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5b2AR-SPARK TF component
104843pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5b2AR-SPARK TF component
104844pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5b2AR-SPARK TF component
104845pAAV-beta-arrestin2-HA-TEV219b2AR-SPARK protease component
104846pLX208-beta-arrestin2-HA-TEV219b2AR-SPARK protease component

Antibodies from Article