Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling.
Liu H, Dong P, Ioannou MS, Li L, Shea J, Pasolli HA, Grimm JB, Rivlin PK, Lavis LD, Koyama M, Liu Z
Proc Natl Acad Sci U S A. 2017 Dec 28. pii: 1713895115. doi: 10.1073/pnas.1713895115. PubMed Article
Liu H, Dong P, Ioannou MS, Li L, Shea J, Pasolli HA, Grimm JB, Rivlin PK, Lavis LD, Koyama M, Liu Z
Proc Natl Acad Sci U S A. 2017 Dec 28. pii: 1713895115. doi: 10.1073/pnas.1713895115. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106300 | PB-hSyn-mSyp-RT1-24xGCN4 | The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4. |
| 106303 | pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE | The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging |
| 106309 | PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B | The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging. |