Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing.
Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA
Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. PubMed Article
Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA
Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107317 | pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS | Expresses SaCas9 in mammalian cells |
| 107318 | pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS | Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells |
| 107319 | pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS | Expresses SpCas9 fused to dSaCas9 in mammalian cells |
| 107320 | pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS | Expresses SpCas9 fused to SaCas9 in mammalian cells |
| 107321 | pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS | Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells |
| 107322 | pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS | Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells |
| 107323 | pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS | Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells |
| 107324 | pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS | Expresses SpCas9 fused to NmCas9 in mammalian cells |
| 107325 | pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS | Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells |
| 107326 | pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS | Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells |
| 107327 | pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS | Expresses SpCas9 fused to dNmCas9 in mammalian cells |
| 107328 | pBluescript_VEGFA-TS3_SpCas9 | U6 driven SpCas9 sgRNA expression for VEGFA site 3 |
| 107329 | pBluescript_VEGFA-TS3_NmCas9 | U6 driven NmCas9 sgRNA expression for VEGFA site 3 |
| 107330 | pBluescript_VEGFA-TS3_SaCas9_sgRNA2 | U6 driven SaCas9 sgRNA expression for VEGFA site 3 |
| 122089 | SpCas9_sgRNA_expression_in_pBluescript | U6 driven SpCas9 sgRNA expression vector for cloning own guides |
| 122090 | SaCas9_sgRNA_expression_in_pBluescript | U6 driven SaCas9 sgRNA expression vector for cloning own guides |
| 122091 | NmCas9_sgRNA_expression_in_pBluescript | U6 driven NmCas9 sgRNA expression vector for cloning own guides |