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Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).
Maass PG, Barutcu AR, Shechner DM, Weiner CL, Mele M, Rinn JL
Nat Struct Mol Biol. 2018 Jan 8. pii: 10.1038/s41594-017-0015-3. doi: 10.1038/s41594-017-0015-3.
PubMed

Plasmids from Article

ID Plasmid Purpose  
107605pUBC_HA_PP7-mCherryTransient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
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107606pUBC_V5_MS2-mVenusTransient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
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107607pUBC_hsPUM1-mCherryTransient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoter
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107608pU6_T7_INT[6xminimal_Puf1]Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter
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107609pUBC_hsPUM1-iRFP670Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter
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111268pUBC_HA_PP7-mVenusTransient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
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