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Artificial Self-Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity.
Haga T, Hirakawa H, Nagamune T
Biotechnol J. 2018 Dec;13(12):e1800088. doi: 10.1002/biot.201800088. Epub 2018 Aug 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108188pET15T+1XPCNA1-PdX fusion protein
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108189pET15b+1(G108C)XExpresses PCNA1(G108C)-PdX fusion protein in E. coli
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108191pET15b+R2(L171C)XExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli
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108192pET15b+2(L171C)XExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli
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108193pET28b+3(R112C/T180C)CExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli
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108194pET28b+R3(R112C/T180C)CExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli
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108195pET28b+R3(R112C/T180C)Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli
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108196pET15b+C3(R112C/T180C)XExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli
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108239pET15b+R2(L171C)Expresses PdR-PCNA2(L171C) fusion protein in E. coli
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Antibodies from Article