T7 vectors with modified T7lac promoter for expression of proteins in Escherichia coli.
Peranen J, Rikkonen M, Hyvonen M, Kaariainen L
Anal Biochem. 1996 May 1;236(2):371-3. PubMed
Peranen J, Rikkonen M, Hyvonen M, Kaariainen L
Anal Biochem. 1996 May 1;236(2):371-3. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112580 | pBAT4 | Used for expression of protein in E. coli as non-fusions |
| 112581 | pBAT5 | Used for expression of protein in E. coli as non-fusions without initiation ATG |
| 112582 | pHAT | Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions |
| 112583 | pHAT2 | Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions |
| 112584 | pHAT3 | Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag fusions |
| 112585 | pHAT4 | Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions |
| 112586 | pHAT5 | Used for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusions |
| 112587 | pGAT | Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence |
| 112588 | pGAT2 | Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions |
| 112589 | pGAT3 | Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions |
| 112590 | pMAT9 | Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP. |
| 112591 | pMAT10 | Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions. Like pMAT9, but without the second BamHI site in the polylinker |
| 112592 | pMAT11 | Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP. |
| 112593 | pTAT5 | Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusions |
| 112594 | pTAT6 | Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusions |