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Engineered promoters enable constant gene expression at any copy number in bacteria.
Segall-Shapiro TH, Sontag ED, Voigt CA
Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
109233pTHSSe_1Empty AmpR pSC101 vector
109234pTHSSe_2Empty SpecR p15A vector
109235pTHSSe_31PUPsp1 GFP reporter
109236pTHSSe_32PUPsp2 GFP reporter
109237pTHSSe_37PTac driving GFP
109238pTHSSe_39PTac driving TALEsp1
109239pTHSSe_40PTac driving TALEsp2
109240pTHSSe_42pSC101 BbsI cloning vector
109241pTHSSe_43pSC101 var1 BbsI cloning vector
109242pTHSSe_44pSC101 var2 BbsI cloning vector
109243pTHSSe_45pSC101 var3 BbsI cloning vector
109244pTHSSe_46pSC101 var4 BbsI cloning vector
109245pTHSSe_47pSC101 var5 BbsI cloning vector
109246pTHSSe_48pSC101 var6 BbsI cloning vector
109247pTHSSe_49pSC101 var7 BbsI cloning vector
109248pTHSSe_50incW BbsI cloning vector
109249pTHSSe_51p15A BbsI cloning vector
109250pTHSSe_52ColE1 BbsI cloning vector
109251pTHSSe_53pUC BbsI cloning vector
109253pTHSSe_59TALEsp1 stabilized promoter driving GFP
109254pTHSSe_60TALEsp2 stabilized promoter driving GFP

Antibodies from Article