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Burden-driven feedback control of gene expression.
Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, Ellis T
Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109380pD864-LacZRhamnose inducible LacZ expression. Used to test burden response in E. coli
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109381pSB1C3-H3 Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli
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109382 pLys-M1Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli
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109383pSB1C3-LuxArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli
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109384pD864-LacZ backboneEmpty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR
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109386pLys-M1 backboneEmpty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR
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109387htpG1-GFP GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
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109388htpG2-GFPGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
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109389 groSL-GFPGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
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109390 ibpAB-GFPGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
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109391Biomolecular FeedBack (FB)This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
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109392DH10GFPDH10B with a "capacity monitor" inserted in the lambda phage integration site.
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109393MG1655GFPMG1655 with a "capacity monitor" inserted in the lambda phage integration site.
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