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Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli.
Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
106250pLibacceptorV2Recombination-mediated cassette exchange with target genomic loci
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110241E. coli K-12 MG1655_rLP1(atpI-gidB)E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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110242E. coli K-12 MG1655_fLP2(yieN-trkD)E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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110243E. coli K-12 MG1655_fLP3(ybbD-ylbG)E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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110244E. coli K-12 MG1655_fLP4(essQ-cspB)E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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110245E. coli K-12 MG1655_rLP5(nth-ydgR)E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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110246E. coli K-12 MG1655_rLP6(ygcE-ygcF)E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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