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Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110573pBTK001Broad-host-range toolkit (BTK) entry vector, with superfolder GFP
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110574pBTK102BTK Type 2 promoter for golden gate assembly
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110575pBTK107BTK Type 2 promoter for golden gate assembly
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110576pBTK110BTK Type 2 promoter for golden gate assembly
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110577pBTK112BTK Type 2 promoter for golden gate assembly
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110578pBTK113BTK Type 2 promoter for golden gate assembly
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110581pBTK121BTK Type 2 promoter for golden gate assembly
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110582pBTK103BTK Type 2 promoter for golden gate assembly
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110583pBTK124BTK Type 2 promoter for golden gate assembly
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110584pBTK130BTK Type 2 promoter for golden gate assembly
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110587pBTK205BTK Type 3 coding sequence for golden gate assembly
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110588pBTK206BTK Type 3 coding sequence for golden gate assembly
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110590pBTK224BTK Type 3 coding sequence for golden gate assembly
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110591pBTK229BTK Type 3 coding sequence for golden gate assembly and homologous recombination
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110592pBTK300BTK Type 4 terminator for golden gate assembly
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110593pBTK301BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)
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110594pBTK305BTK Type 4 terminator for golden gate assembly
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110595pBTK211BTK Type 6 Repressor CDS for golden gate assembly
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110597pBTK401BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly
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110598pBTK402BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly
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110599pBTK403BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly
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110600pBTK599BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination
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110601pBTK599sBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination
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110602pBTK501BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)
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110603pBTK519BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)
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110604pBTK520BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)
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110605pBTK527BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)
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110606pBTK527aBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)
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110607pBTK601BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)
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110608pBTK614BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)
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110609pBTK615BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9
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