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Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110624pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNAA single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
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110625MSP1594_2x_NLSMammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)
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110626U6_sgRNA_CAG_hSt1Cas9_LMD9A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
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110627St1Cas9_LMD-9_sgRNA_pUC19U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).
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136651U6_sgRNA_CAG_hSt1Cas9_CNRZ1066A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.
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136652U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHAExpresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
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136653U6_sgRNA_CAG_hSt1Cas9_LMG18311A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
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136654U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHAExpresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
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136655U6_sgRNA_CAG_hSt1Cas9_TH1477A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
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136656U6_sgRNA_CAG_hSt1Cas9_MTH17CL396A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
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136657U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHAExpresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
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136659U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHAExpresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
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136660U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA
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Antibodies from Article