Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. PubMed Article
Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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110624 | pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA | A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA |
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110625 | MSP1594_2x_NLS | Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) |
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110626 | U6_sgRNA_CAG_hSt1Cas9_LMD9 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA. |
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110627 | St1Cas9_LMD-9_sgRNA_pUC19 | U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9). |
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136651 | U6_sgRNA_CAG_hSt1Cas9_CNRZ1066 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA. |
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136652 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA | Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA |
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136653 | U6_sgRNA_CAG_hSt1Cas9_LMG18311 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA. |
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136654 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA | Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA |
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136655 | U6_sgRNA_CAG_hSt1Cas9_TH1477 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA. |
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136656 | U6_sgRNA_CAG_hSt1Cas9_MTH17CL396 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA. |
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136657 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA | Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA |
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136659 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA | Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA |
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136660 | U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9 | Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA |
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