Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055. PubMed Article
Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84524 | pET30a-SRAP | Expresses murine SRAP domain (250 aa) in bacteria |
| 84567 | p3XFlag-YedK | Expresses YedK in mammalian cells |
| 84568 | pET30a-SRAP-delCys2 | Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2 |
| 86884 | pET30a-YedK | Bacterial expression of E. coli SRAP ortholog YedK |
| 89691 | pGEX4T-1 Srap1 | Expresses murine Srap1 in bacteria |