Cryo-EM shows how dynactin recruits two dyneins for faster movement.
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462. PubMed Article
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111657 | His-ZZ-TEV-BICDR1-GFP | C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ. |
| 111857 | His-ZZ-TEV-SNAPf-BICD2N(1-400) | N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ. |
| 111858 | His-ZZ-TEV-SNAPf-BICDR1 | N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ. |
| 111859 | His-ZZ-TEV-BICDR1 | Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ. |
| 111860 | Hook3(1-522)-SNAPf-Psc-StrepII | C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag. |
| 111861 | Hook3(1-522)-GFP-Psc-StrepII | C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag. |
| 111862 | His-ZZ-TEV-BICDN(1-400)-GFP | C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ. |