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New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa MI, Bartel DP, Fink GR
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111430pV1393CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
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111431pV1524CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
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111434pV1539Genedrive entry vector for Candida albicans
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111435pV1326S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
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111436pV1382S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
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111438pV1329Solo vector pV1326 +sgCgADE2
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111439pV1435Solo vector pV1382 + sgCgADE2
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111440pV1464N. castellii Solo CRISPR vector, marked with URA3 and NatR
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111443pV1338Solo vector pV1326 + sgScADE2
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111444pVG1Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate
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111445pV1386Solo vector pV1382 + sgScADE2
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