Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
p62 filaments capture and present ubiquitinated cargos for autophagy.
Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S
EMBO J. 2018 Mar 1;37(5). pii: embj.201798308. doi: 10.15252/embj.201798308. Epub 2018 Jan 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
169168 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B) Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
190191pET-Duet1-His-TEV-mCherry-OPTN WTPlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797
190192pET-Duet1-6xHis-TEV-OPTNPlasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397
190194pET-Duet1-6xHis-TEV-NDP52Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

Antibodies from Article