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Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.
Jenni S, Harrison SC
Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111981pACY.HRF_T7_term_HR1Sub cloning vector position 1
111983pACY.HR1_T7_term_HR2Subcloning vector position 2
111984pACY.HR2_T7_term_HR3Subcloning vector position 3
111985pACY.HR3_T7_term_HR4Subcloning vector position 4
111986pACY.HR4_T7_term_HRRSubcloning vector position 5
112011pAA3.stelNExpresses TelN enzyme
112025pAA.3.lacIh_I12X86Expresses LacI_I12X86 protein
112149peAA.11"Vector AA"
112150peDK.11"Vector DK"
112151jk089_h12C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Antibodies from Article