CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
Swings T, Marciano DC, Atri B, Bosserman RE, Wang C, Leysen M, Bonte C, Schalck T, Furey I, Van den Bergh B, Verstraeten N, Christie PJ, Herman C, Lichtarge O, Michiels J
Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5. PubMed
Swings T, Marciano DC, Atri B, Bosserman RE, Wang C, Leysen M, Bonte C, Schalck T, Furey I, Van den Bergh B, Verstraeten N, Christie PJ, Herman C, Lichtarge O, Michiels J
Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113398 | pKDsgRNA-FRT | expresses gRNA for Cas9 FRT targetting |
| 113633 | pCas-CmR(+) | Chloramphenicol resistant derivative of pCas plasmid |
| 113637 | pTargetF-FRT | Derivative of pTargetF with gRNA targeting FRT site |