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Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins.
Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ
MBio. 2018 Dec 4;9(6). pii: mBio.02321-18. doi: 10.1128/mBio.02321-18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113434pEJS540-pCSDest2-AcrIIC4HpaExpresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
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113435pEJS542-pCSDest2-AcrIIC5SmuExpresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells
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113436pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLSExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
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113437pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLSExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells
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113438pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRESExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells
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113439pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRESExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cells
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113440pEJS663-pMCSG7-AcrIIC4HpaExpresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells
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113441pEJS664-pMCSG7-AcrIIC5SmuExpresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells
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121540pEJS1026-pMCSG7-HpaCas9Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells
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121541pEJS1027-pMCSG7-SmuCas9Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells
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