Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins.
Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ
MBio. 2018 Dec 4;9(6). pii: mBio.02321-18. doi: 10.1128/mBio.02321-18. PubMed Article
Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ
MBio. 2018 Dec 4;9(6). pii: mBio.02321-18. doi: 10.1128/mBio.02321-18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113434 | pEJS540-pCSDest2-AcrIIC4Hpa | Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells |
| 113435 | pEJS542-pCSDest2-AcrIIC5Smu | Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells |
| 113436 | pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS | Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells |
| 113437 | pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS | Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells |
| 113438 | pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES | Expresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells |
| 113439 | pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES | Expresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cells |
| 113440 | pEJS663-pMCSG7-AcrIIC4Hpa | Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells |
| 113441 | pEJS664-pMCSG7-AcrIIC5Smu | Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells |
| 121540 | pEJS1026-pMCSG7-HpaCas9 | Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells |
| 121541 | pEJS1027-pMCSG7-SmuCas9 | Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells |