BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis synthetic biology
Wicke N, Radford D, Verrone V, Wipat A, French CE
bioRxiv 185108
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112775 | pBP-P43 | Strong promoter activity during exponential and stationary phase |
| 112776 | pBP-Pveg | Strong promoter active during sporulation and vegetative growth |
| 112777 | pBP-PliaG | Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage |
| 112778 | pBP-Pspac | Promoter repressed by LacI (not natively produced in B. subtilis 168) |
| 112779 | pBP-Pars | Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite |
| 112780 | pBP-ORF-xylE | Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0. |
| 112781 | pTU1-A-amyEU | Upstream homology arm, amyE encodes _-amylase |
| 112782 | pTU1-D-amyED | Downstream homology arm, amyE encodes _-amylase |
| 112783 | pTU1-A-recJU | Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance |
| 112784 | pTU1-D-recJD | Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance |
| 112785 | pTU1-A-rrnOU | Upstream homology arm, rrnO-23S (23S rRNA) |
| 112786 | pTU1-D-rrnOD | Downstream homology arm, rrnO-23S (23S rRNA) |
| 112787 | pTU1-A-spsCU | Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis |
| 112788 | pTU1-D-spsCD | Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis |
| 112789 | pTU1-A-yckABU | Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis |
| 112790 | pTU1-D-yckABD | Downstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis |
| 112791 | pTU1-C-cat | Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase) |
| 112792 | pTU2-AmyE-Pveg-eGFP | Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation |