Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint.
Etemad B, Kuijt TE, Kops GJ
Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. PubMed Article

Plasmids from Article

ID Plasmid Purpose
114021pLV-CMV-tagRFP-Mad2Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter
114022pcDNA5-puro-eGFP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
114023pcDNA5-puro-eGFP-HEC1-9AInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
114025pcDNA5-puro-eGFP-HEC1-d80Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
114026pcDNA5-hygro-eGFP-STOP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
114027pcDNA5-hygro-eGFP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
114028pcDNA5-hygro-eGFP-HEC1-9AInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
114029pcDNA5-hygro-eGFP-HEC1-9DInducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines
114030pcDNA5-hygro-eGFP-HEC1-d80Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
114031pcDNA5-hygro-eGFP-HEC1-8A/S15DInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines
114032pcDNA5-hygro-eGFP-HEC1-8A/S55DInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Antibodies from Article