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Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint.
Etemad B, Kuijt TE, Kops GJ
Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114021pLV-CMV-tagRFP-Mad2Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter
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114022pcDNA5-puro-eGFP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
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114023pcDNA5-puro-eGFP-HEC1-9AInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
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114025pcDNA5-puro-eGFP-HEC1-d80Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
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114026pcDNA5-hygro-eGFP-STOP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
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114027pcDNA5-hygro-eGFP-HEC1Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
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114028pcDNA5-hygro-eGFP-HEC1-9AInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
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114029pcDNA5-hygro-eGFP-HEC1-9DInducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines
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114030pcDNA5-hygro-eGFP-HEC1-d80Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
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114031pcDNA5-hygro-eGFP-HEC1-8A/S15DInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines
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114032pcDNA5-hygro-eGFP-HEC1-8A/S55DInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines
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Recombinant Antibodies from Article