Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates.
Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H
Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070. PubMed Article
Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H
Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114113 | pCDNA3-PKA-RIIβ-sREACh | Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh. |
| 114114 | pCDNA3_PKA-CαN-mPAGFP | Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114130 | pCDNA3_nmPKA-CαN-mPAGFP | Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114131 | pCDNA3_nmPKA-Cα-mPAGFP | Mammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114132 | pCDNA3_altPKA-Cα-mEGFP | Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. |
| 114133 | pCDNA3_altPKA-Cα-mPAGFP | Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114134 | pCDNA3_PKA-Cα(S11A)-mPAGFP | Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114135 | pCDNA3_PKA-Cα(S11D)-mPAGFP | Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP. |
| 114136 | pEGFP_mEGFP-GluA1c | Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP. |
| 114138 | pCAGGS_cyt-AKAR5 | Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 114139 | pCAGGS_m-AKAR5 | Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 114140 | pCAGGS_m-AKAR5’ | Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM |
| 114141 | pCAGGS_AKAR5’ | Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 114142 | pEFU6-EGFP_shPKA-Cα | Expression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed. |
| 114143 | pCDNA3_shRNA resistant PKA-Cα-mEGFP | Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down. |
| 114144 | pCDNA3_shRNA resistant nmPKA-Cα-mEGFP | The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down. |