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Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates.
Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H
Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114113pCDNA3-PKA-RIIβ-sREAChMammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.
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114114pCDNA3_PKA-CαN-mPAGFPMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
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114130pCDNA3_nmPKA-CαN-mPAGFPMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
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114131pCDNA3_nmPKA-Cα-mPAGFPMammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
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114132pCDNA3_altPKA-Cα-mEGFPAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.
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114133pCDNA3_altPKA-Cα-mPAGFPAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
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114134pCDNA3_PKA-Cα(S11A)-mPAGFPMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
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114135pCDNA3_PKA-Cα(S11D)-mPAGFPMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
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114136pEGFP_mEGFP-GluA1cMammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.
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114138pCAGGS_cyt-AKAR5Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
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114139pCAGGS_m-AKAR5Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
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114140pCAGGS_m-AKAR5’Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
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114141pCAGGS_AKAR5’Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
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114142pEFU6-EGFP_shPKA-CαExpression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed.
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114143pCDNA3_shRNA resistant PKA-Cα-mEGFPMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
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114144pCDNA3_shRNA resistant nmPKA-Cα-mEGFPThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
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