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MicroRNA-223 Suppresses the Canonical NF-kappaB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation.
Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, Freeman JL, Kasinski AL, Deng Q
Cell Rep. 2018 Feb 13;22(7):1810-1823. doi: 10.1016/j.celrep.2018.01.058.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
97142miR-223-RFP pCDNAexpression of miR-223 and TagRFP
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97143miR-vector-RFP pCDNAfor expression of miRNA and TagRFP
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97144Tol2-lyzC-Gal4-Vp16-Crys-CFPFor Gal4/UAS expression system
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97145Tol2-UAS-Dendra2For Gal4/UAS expression system
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97146Tol2-UAS-Dendra2-miR-223 spongeFor miR-223 knock down
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97147Tol2-lyzC-RFP-miR-30 backboneAs a control of miRNA expression
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97148Tol2-lyzC-RFP-miR-223miR-223 expression
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97149Tol2-krt4-RFPAs a control of miRNA sponge
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97150Tol2-krt4-RFP-miR-223 spongeFor miR-223 knock down
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97151Tol2-krt4-GFPlabel apical epithelial cells in zebrafish
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97152pSI-check2-Cul1a-3'UTRFor dual luciferase report assay
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97153pSI-check2-Cul1b-3'UTRFor dual luciferase report assay
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97154pSI-check2-Traf6-3'UTRFor dual luciferase report assay
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97155pSI-check2-Tab1-3'UTRFor dual luciferase report assay
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97156pSI-check2-CUL1-3'UTRFor dual luciferase report assay
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97157pSI-check2-TAB2-3'UTRFor dual luciferase report assay
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106979pSI-Check2-hRluc-NFkB-fireflyLuciferase reporter with internal control for NFkB signal.
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Antibodies from Article