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Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630.
DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119879pDD120Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.
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119880pDD143Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.
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119882pDD57Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.
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119883pDD56Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.
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119884pDD108Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker
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119885pDD132Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker
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119893pDD112pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630
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