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A role for mitotic bookmarking of SOX2 in pluripotency and differentiation.
Deluz C, Friman ET, Strebinger D, Benke A, Raccaud M, Callegari A, Leleu M, Manley S, Suter DM
Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
115685pKISox1-P2A-eGFP-HygroKnock-in of P2A-eGFP at the mouse Sox1 gene
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115686pKIBra-P2A-mCherry-Puro-sfGFPKnock-in of P2A-mCherry at the mouse Brachyury (T) gene
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115687pLV-PGK-SNAP-MD-SOX2Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)
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115688pLV-PGK-SNAP-MDMUT-SOX2Constitutive expression of a SOX2 protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control
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