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BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts.
Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC
ACS Synth Biol. 2019 May 7. doi: 10.1021/acssynbio.8b00381.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117048pJL1-neoRIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoter
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117050pJL1-aadAIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoter
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117051pJL1-SpCas9In vitro expression of S. pyogenes Cas9 from the T7 promoter
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117052pJL1-mRFP1 gRNAIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter
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