CRISPR-KO screen identifies Dmap1 as a regulator of chemically-induced reprogramming and differentiation of cardiac progenitors.
Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012. PubMed Article
Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117135 | pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5 | Expression vector for Nkx2-5 and gRNA |
| 117137 | pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5 | Expression vector encoding BFP-BSD-Nkx2-5 |
| 117139 | pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM) | Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP |
| 117141 | pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP | Express gRNA vs Dmap1 |
| 117143 | pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP | Express gRNA vs Dmap1 |
| 117144 | pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP | Express gRNA vs Dmap1 |