Use of mutant 125I-perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells.
Das A, Goldstein JL, Anderson DD, Brown MS, Radhakrishnan A
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10580-5. doi: 10.1073/pnas.1309273110. Epub 2013 Jun 10. PubMed Article
Das A, Goldstein JL, Anderson DD, Brown MS, Radhakrishnan A
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10580-5. doi: 10.1073/pnas.1309273110. Epub 2013 Jun 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120400 | pPFO* | Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C. |