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Golden Gate based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica.
Egermeier M, Sauer M, Marx H
FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 10.1093/femsle/fnz022. PubMed Article

Plasmids from Article

ID Plasmid Purpose
117819BB1_12_YlpTEFPromoter pTEF of Y. lipolytica for GoldenMOCS
117820BB1_12_YlpGPDPromoter pGPD of Y. lipolytica for GoldenMOCS
117821BB1_34_YlCyc1TTTranscription terminator Cyc1TT of Y. lipolytica for GoldenMOCS
117822BB1_34_YlMig1TTTranscription terminator Mig1TT of Y. lipolytica for GoldenMOCS
117823BB1_23_YlGUT1Encodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS
117824pMEG_BB2_YL001Transcription unit of Y. lipolytica for GoldenMOCS
117825pMEG_BB3_YL001Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica
117826pMEG_YLCas9_emptyCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica
117827pMEG_BB3_YL18H_ABGoldenMOCS backbone for 1 TU in Y. lipolytica
117828pMEG_BB3_YL18H_ACGoldenMOCS backbone for 2 TU in Y. lipolytica
117829pMEG_BB3_YL68N_ABGoldenMOCS backbone for 1 TU in Y. lipolytica
117830pMEG_BB3_YL68N_ACGoldenMOCS backbone for 2 TU in Y. lipolytica
117831pMEG_BB3_YL68N_ADGoldenMOCS backbone for 3 TU in Y. lipolytica
117832pMEG_BB3_YL68N_AEGoldenMOCS backbone for 4 TU in Y. lipolytica

Antibodies from Article